Monitoring Of Vaccine Take By Quantitative Real- Time Polymerase Chain Reaction Following Different Marek’s Disease Vaccination Programs In Future Broiler Breeders

Delvecchio A., Gimeno I. & Lemiere S. 


The objective of this study was to compare different Marek’s disease (MD) vaccination programs in conventional broiler breeder pullets utilizing quantitative real-time polymerase chain reaction (qPCR) on feather pulp and spleen samples. Sample collection utilized a novel transport system of antigenic material from the desired samples. Vaccine programs compared in ovo, subcutaneous at hatch or intramuscular at hatch injection of a recombinant Marek’s and IBD vaccine associated to a MD Rispens vaccine, and injected twice as part of broiler breeder vaccination program. Double vaccinations using VAXXITEK® HVT+IBD and Rispens strain mixed vaccines did not impair the vaccine virus replication kinetics in vivo.