Interactions Between Live Newcastle Disease, Avian Metapneumovirus And Infectious Bronchitis Vaccine Viruses In Specific-Pathogen-Free Chicks
S. LEMIERE, F. AWAD, A. FORRESTER, M. BAYLIS, R. JONES and K. GANAPATHY
Interactions between live avian metapneumovirus (aMPV), Newcastle disease virus (NDV) and infectious bronchitis virus (IBV) vaccines following simultaneous vaccination of one day old specifie pathogens free chicks were evaluated. Chicks were divided into eight groups. One group served as unvaccinated control. Other seven groups were vaccinated against NDV, aMPV or/and IBV (single, dual, and triple). At intervals, oropharyngeal (OP) swabs, blood and tissues were collected for virus detection by reverse transcriptase polymerase chain reaction (RT-PCR). At 21 day post vaccination (dpv), birds were bled and 10 chicks from each group were challenged with virulent IBV or aMPV. Findings from this study showed that based on the absence of clinical signs following vaccinations and protection conferred against IBV M41 and virulent aMPV subtype B, it appears that these vaccines can be applied simultaneously. The study also demonstrated that chicks that had not received aMPV vaccination showed substantial clinical signs when challenged at 3 weeks old.
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