IMPORTANT: If you reside in a country where health product communications are restricted to health care professionals, and you are NOT a health care professional, please contact your veterinarian instead of reading the following information.


Because vaccination with VAXXITEK HVT + IBD involves just a single injection, proper handling and administration are critical to ensure successful immunization. Our Vaccination Technologies and Services (VTS) group delivers the vaccine to your site, provides the necessary equipment and supplies, and trains and supports hatchery staff. Here's an overview of the process... 

Cold chain integrity

VAXXITEK HVT + IBD is a frozen vaccine product, so maintaining the cold chain is vital. Our VTS technicians deliver the vaccine ampoules in liquid nitrogen dewars and provide detailed instructions for ongoing storage and handling. Most importantly:

  • Continue storing VAXXITEK HVT + IBD ampoules in liquid nitrogen until ready for use
  • Check liquid nitrogen levels in the dewars daily
  • Store diluent bags separately, at room temperature

Mixing & preparation

VAXXITEK HVT + IBD is supplied in frozen ampoules that each contain 1,000 doses when used in healthy day-old chicks (subcutaneous injection) or 4,000 doses when used in-ovo (at 18-19 days of embryonation). Each ampoule is thawed and added to a bag of diluent in order to reconstitute the vaccine at a rate of 0.05 or 0.1 mL per dose for in-ovo application depending upon the technology, and at a rate of 0.2 mL per dose for subcutaneous injection at one day of age. We also provide an optional blue dye, which can be added to the diluent mixture to help monitor quality of subcutaneous injection. 

Our VTS team provides all necessary supplies—from the vaccine, diluent, and dye, to disposable equipment, instructional mixing mats, and more—to ensure the process goes smoothly. 

You'll also find complete mixing, preparation, and administration instructions in the product label.

Hatchery administration: procedures and dosing

Once the vaccine-diluent mixture is prepared, it should be used within one hour. Injection machinery should be set to deliver the following quantities of the vaccine-diluent mixture: 

  •  In-ovo (18-19 days)0.05 ml or 0.1 mL per egg
  • Subcutaneous: 0.2 ml per healthy day-old chick

Our VTS group develops innovative vaccination machinery for both types of hatchery administration, and will adjust settings and provide ongoing equipment maintenance.

Precautions & guidelines

  • At all steps of the process, follow good sanitation, sterilization, and biosecurity practices
  • Use protective equipment, especially when handling liquid nitrogen
  • Work quickly, with an awareness of temperature issues, but also carefully
  • Do not vaccinate broiler birds with VAXXITEK HVT + IBD within 21 days of slaughter
  • Do not vaccinate diseased embryonated eggs or diseased birds

And remember: contact your Merial VTS associate at any time if you need assistance using VAXXITEK HVT + IBD—we're committed to your vaccination success.